L-GLUTAMINE SUPPLEMENTATION ASSOCIATED WITH MODERATE AEROBIC TRAINING IMPROVES BIOMETRIC, GLYCEMIC PROFILE AND THE ANTIOXIDANT DEFENSE

Authors

DOI:

https://doi.org/10.21527/2176-7114.2020.39.62-77

Keywords:

glucose intolerance, oxidative stress, HSP70, skeletal muscle, adiposity

Abstract

Introduction: L-glutamine is a non-essential amino acid, whose intrinsic pool of appears to be depleted during catabolic conditions, such as intense or high duration exercise, and to avoid the exercise- related benefits. Therefore, its supplementation could provide an additional source of L-glutamine and prevent these effects. However, the oral intake of its free form has been discouraged, despites of some evidences reporting positive effects. Objective: to verify whether the L-glutamine supplementation (in its free form) could provide an additional improvement in biometric, glycemic and redox parameters, in animals undergoing moderate aerobic training (MAT). Methods: 28 Swiss male mice were divided into four groups: Cont (n=7), Ex (n=7), Glut (n=8), and Ex+Glut (n=6). Glut and Ex+Glut received gastric gavage of L-glutamine (1g/kg), while Cont and Ex groups received 100 µL of PBS one hour before exercising, five days/week, six weeks. Ex and Ex+Glut underwent moderate swimming, while Cont and Glut remained sedentary, for the same period. Mice started swimming with 2% of body weight attached to the tail during 20 min, and ended the experiment with 4% during 60 min. Results: L-glutamine supplementation increased the gastrocnemius mass and improved the glucose tolerance in animals submitted to MAT. It improved the antioxidant status in gastrocnemius, liver and pancreas, and declined it in adipose tissue in animals undergoing MAT. The drop of adipose antioxidant defense was associated with adiposity, while pancreas antioxidant activity was inversely associated with the glucose intolerance. Conclusion: L-glutamine (free form) improves biometric and glucose parameters, and enhances antioxidant activities.

Author Biographies

Analú Bender dos Santos, Universidade Regional do Noroeste do Estado do Rio Grande do Sul

Research Group in Physiology, Department of Life Sciences, Regional University of Northwestern Rio Grande do Sul State (UNIJUI), Rua do Comércio, 3000 – Bairro Universitário – Ijuí - RS, Brazil, 98700-000, Phone: +55 (55) 3332-0476.

Postgraduate Program in Integral Attention to Health (PPGAIS-UNIJUÍ/UNICRUZ), Ijuí, RS, Brazil

Lílian Corrêa Costa-Beber

Research Group in Physiology, Department of Life Sciences, Regional University of Northwestern Rio Grande do Sul State (UNIJUI), Rua do Comércio, 3000 – Bairro Universitário – Ijuí - RS, Brazil, 98700-000, Phone: +55 (55) 3332-0476.

Postgraduate Program in Integral Attention to Health (PPGAIS-UNIJUÍ/UNICRUZ), Ijuí, RS, Brazil

Eloisa Gabriela de Pelegrin Basso

Research Group in Physiology, Department of Life Sciences, Regional University of Northwestern Rio Grande do Sul State (UNIJUI), Rua do Comércio, 3000 – Bairro Universitário – Ijuí - RS, Brazil, 98700-000, Phone: +55 (55) 3332-0476.

Atmospheric Pollution Laboratory, Postgraduate Program in Health Sciences, Federal University of Health Sciences of Porto Alegre (UFCSPA), Rua Sarmento Leite, 245, Porto Alegre, RS, Brazil

Pauline Brendler Goettems Fiorin

Research Group in Physiology, Department of Life Sciences, Regional University of Northwestern Rio Grande do Sul State (UNIJUI), Rua do Comércio, 3000 – Bairro Universitário – Ijuí - RS, Brazil, 98700-000, Phone: +55 (55) 3332-0476.

Atmospheric Pollution Laboratory, Postgraduate Program in Health Sciences, Federal University of Health Sciences of Porto Alegre (UFCSPA), Rua Sarmento Leite, 245, Porto Alegre, RS, Brazil

Renan Daniel Bueno Basso

Research Group in Physiology, Department of Life Sciences, Regional University of Northwestern Rio Grande do Sul State (UNIJUI), Rua do Comércio, 3000 – Bairro Universitário – Ijuí - RS, Brazil, 98700-000, Phone: +55 (55) 3332-0476.

Postgraduate Program in Integral Attention to Health (PPGAIS-UNIJUÍ/UNICRUZ), Ijuí, RS, Brazil

Maicon Machado Sulzbacher

Research Group in Physiology, Department of Life Sciences, Regional University of Northwestern Rio Grande do Sul State (UNIJUI), Rua do Comércio, 3000 – Bairro Universitário – Ijuí - RS, Brazil, 98700-000, Phone: +55 (55) 3332-0476.

Postgraduate Program in Integral Attention to Health (PPGAIS-UNIJUÍ/UNICRUZ), Ijuí, RS, Brazil

Mirna Stela Ludwig, Universidade Regional do Noroeste do Estado do Rio Grande do Sul

Research Group in Physiology, Department of Life Sciences, Regional University of Northwestern Rio Grande do Sul State (UNIJUI), Rua do Comércio, 3000 – Bairro Universitário – Ijuí - RS, Brazil, 98700-000, Phone: +55 (55) 3332-0476.

Postgraduate Program in Integral Attention to Health (PPGAIS-UNIJUÍ/UNICRUZ), Ijuí, RS, Brazil

Thiago Gomes Heck, Universidade Regional do Noroeste do Estado do Rio Grande do Sul

Research Group in Physiology, Department of Life Sciences, Regional University of Northwestern Rio Grande do Sul State (UNIJUI), Rua do Comércio, 3000 – Bairro Universitário – Ijuí - RS, Brazil, 98700-000, Phone: +55 (55) 3332-0476.

Postgraduate Program in Integral Attention to Health (PPGAIS-UNIJUÍ/UNICRUZ), Ijuí, RS, Brazil

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Published

2020-10-22

How to Cite

Bender dos Santos, A., Corrêa Costa-Beber, L., de Pelegrin Basso, E. G., Brendler Goettems Fiorin, P., Bueno Basso, R. D., Machado Sulzbacher, M., Ludwig, M. S., & Heck, T. G. (2020). L-GLUTAMINE SUPPLEMENTATION ASSOCIATED WITH MODERATE AEROBIC TRAINING IMPROVES BIOMETRIC, GLYCEMIC PROFILE AND THE ANTIOXIDANT DEFENSE. Context and Health Journal, 20(39), 62–77. https://doi.org/10.21527/2176-7114.2020.39.62-77