EFEITO DA SUPLEMENTAÇÃO COM ÓLEO DE PEIXE NO MODELO EXPERIMENTAL DE SEPSE

Abstract

Introduction: Sepsis is a highly prevalent syndrome, that causes high morbidity and mortality. Therapies attached to conventional clinical treatment should be studied in order to minimize the effects of sepsis. Objective: To investigate the effect of supplementation with fish oil (FO) rich in omega-3, on renal and pulmonary function, mortality and oxidative stress in rats with sepsis. Methodology: Sepsis was induced by puncture and cecal ligation (CLP). Omega (1 g/kg/day, orally) was given seven days before CLP. Sham groups (CLP without FO, n=10) and CLP + FO (supplemented with FO, n=10) were studied. Oxidative stress, and pulmonary and renal histology were analyzed, with regard to their structure and presence of polymorphonuclear cells (PMN), pulmonary edema index, renal function and survival in 72 hours. Results: Oxidative stress was not different for CLP + FO group (p=0.06). Histology, regarding structure, showed that the group supplemented with FO had a lower structural damage, both in the lung and kidney tissues, however, the polymorphonuclear cells count (PMN) was not different between the groups, for both systems. The rate of pulmonary edema, although lower in the CLP + FO group, was not significantly different from that found in the Sham group (p=0.59). There were no changes in survival improvement caused by FO supplementation. Renal function, studied by urea and creatinine, was not different between the groups. Conclusion: Fish oil (FO) supplementation, in this study, did not improve renal, pulmonary and mortality conditions in rats with sepsis.